CPT1A Antibody

Product Specifications

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

1374

Swiss Prot

P50416

Accession Number

P50416

Host

Rabbit

Reactivity

Human

Immunogen

This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-756 amino acids from the C-terminal region of human CPT1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

88 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

CPT1A

NCBI Official Name

Carnitine O-palmitoyltransferase 1, liver isoform

NCBI Organism

Homo sapiens

Background Reference 01

Gessner, B.D., et al. Pediatrics 126 (5) :945-951 (2010)

Background Reference 02

Collins, S.A., et al. Mol. Genet. Metab. 101 (2-3), 200-204 (2010) :

Background Reference 03

Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

Background Reference 04

Song, S., et al. Mol. Cell. Endocrinol. 325 (1-2), 54-63 (2010) :

Other Product Names

Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1