MED6 Antibody

Product Specifications

Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

NCBI Gene ID

10001

Swiss Prot

O75586

Accession Number

O75586

Host

Rabbit

Reactivity

Human

Immunogen

This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human MED6.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

28 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MED6

NCBI Official Name

Mediator of RNA polymerase II transcription subunit 6

NCBI Organism

Homo sapiens

Background Reference 01

Pavri, R., et al. Mol. Cell 18 (1) :83-96 (2005)

Background Reference 02

Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)

Background Reference 03

Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (8) :2339-2344 (2004)

Background Reference 04

Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

Other Product Names

Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, ARC33, Mediator complex subunit 6, hMed6, Renal carcinoma antigen NY-REN-28, MED6, ARC33