METAP2 Antibody

Product Specifications

Background

This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.

NCBI Gene ID

10988

Swiss Prot

P50579

Accession Number

P50579

Host

Rabbit

Reactivity

Human

Immunogen

This METAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human METAP2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

53 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

METAP2

NCBI Official Name

Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175}

NCBI Organism

Homo sapiens

Background Reference 01

Xiao, Q., et al. Biochemistry 49 (26) :5588-5599 (2010)

Background Reference 02

Wang, X., et al. PLoS ONE (8), E11934 (2010) :

Background Reference 03

Selvakumar, P., et al. Mol. Cancer 8, 65 (2009) :

Background Reference 04

Warder, S.E., et al. J. Proteome Res. 7 (11) :4807-4820 (2008)

Other Product Names

Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175}, MAP 2 {ECO:0000255|HAMAP-Rule:MF_03175}, MetAP 2 {ECO:0000255|HAMAP-Rule:MF_03175}, 341118 {ECO:0000255|HAMAP-Rule:MF_03175}, Initiation factor 2-associated 67 kDa glycoprotein {ECO:0000255|HAMAP-Rule:MF_03175}, p67 {ECO:0000255|HAMAP-Rule:MF_03175}, p67eIF2 {ECO:0000255|HAMAP-Rule:MF_03175}, Peptidase M {ECO:0000255|HAMAP-Rule:MF_03175}, METAP2 {ECO:0000255|HAMAP-Rule:MF_03175}