Mouse Ern1 Antibody

Product Specifications

Background

Ern1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

NCBI Gene ID

78943

Swiss Prot

Q9EQY0

Accession Number

Q9EQY0

Host

Rabbit

Reactivity

Mouse

Immunogen

This Mouse Ern1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 827-855 amino acids from the C-terminal region of mouse Ern1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

110 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

Ern1

NCBI Official Name

Serine/threonine-protein kinase/endoribonuclease IRE1

NCBI Organism

Mus musculus

Background Reference 01

Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (37) :16113-16118 (2010)

Background Reference 02

Auf, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (35) :15553-15558 (2010)

Background Reference 03

Oikawa, D., et al. FEBS Lett. 584 (5) :1066-1070 (2010)

Background Reference 04

Yang, L., et al. PLoS ONE (7), E11621 (2010) :

Other Product Names

Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, Ern1 {ECO:0000312|MGI:MGI:1930134}