MARCH4 Antibody

Product Specifications

Background

MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19) . MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]) .

NCBI Gene ID

57574

Swiss Prot

Q9P2E8

Accession Number

Q9P2E8

Host

Rabbit

Reactivity

Human

Immunogen

This MARCH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the C-terminal region of human MARCH4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

46 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

MARCH4

NCBI Official Name

E3 ubiquitin-protein ligase MARCH4

NCBI Organism

Homo sapiens

Background Reference 01

Bartee, E., et al. J. Virol. 78 (3) :1109-1120 (2004)

Other Product Names

E3 ubiquitin-protein ligase MARCH4, 632-, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, MARCH-IV, RING finger protein 174, MARCH4, KIAA1399, RNF174