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COX5A Antibody

Product Specifications

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq].

NCBI Gene ID

9377

Swiss Prot

P20674

Accession Number

P20674

Host

Rabbit

Reactivity

Mouse

Immunogen

This COX5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human COX5A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

17 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

COX5A

NCBI Official Name

Cytochrome c oxidase subunit 5A, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Chen, Z.X., et al. Cell Death Differ. 17 (3) :408-420 (2010)

Background Reference 02

Fornuskova, D., et al. Biochem. J. 428 (3) :363-374 (2010)

Background Reference 03

Uddin, M., et al. BMC Evol. Biol. 8, (2008) :

Background Reference 04

Williams, S.L., et al. J. Biol. Chem. 279 (9) :7462-7469 (2004)

Other Product Names

Cytochrome c oxidase subunit 5A, mitochondrial, Cytochrome c oxidase polypeptide Va, COX5A

Tested Applications

WB

Protein ID

218511986

Physical Properties

Liquid

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