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EIF3A Antibody

Product Specifications

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

NCBI Gene ID

8661

Swiss Prot

Q14152

Accession Number

Q14152

Host

Rabbit

Reactivity

Human

Immunogen

This EIF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1219-1247 amino acids from the C-terminal region of human EIF3A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=56-759

Calculated Molecular Weight

167 kDa

Fragment

Rabbit Ig

Symbol

EIF3A

NCBI Official Name

Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}

NCBI Organism

Homo sapiens

Background Reference 01

Enchev, R.I., et al. Structure 18 (4) :518-527 (2010)

Background Reference 02

Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19 (1) :251-257 (2010)

Background Reference 03

Dong, Z., et al. Exp. Cell Res. 315 (11) :1889-1894 (2009)

Background Reference 04

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

Other Product Names

Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3a {ECO:0000255|HAMAP-Rule:MF_03000}, Eukaryotic translation initiation factor 3 subunit 10 {ECO:0000255|HAMAP-Rule:MF_03000}, eIF-3-theta {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3 p167, eIF3 p180, eIF3 p185, EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}

Tested Applications

WB, IHC-P

Protein ID

6685537

Physical Properties

Liquid

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