Mdm2 Antibody

Product Specifications

Background

MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

NCBI Gene ID

4193

Swiss Prot

Q00987

Accession Number

Q00987

Host

Rabbit

Reactivity

Human

Immunogen

This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

55 kDa

Fragment

Rabbit Ig

Symbol

MDM2

NCBI Official Name

E3 ubiquitin-protein ligase Mdm2

NCBI Organism

Homo sapiens

Background Reference 01

Burch, L.R., et al., J. Mol. Biol. 337 (1) :115-128 (2004) . Schon, O., et al., J. Mol. Biol. 336 (1) :197-202 (2004) . Mantesso, A., et al., J. Oral Pathol. Med. 33 (2) :96-101 (2004) . Shmueli, A., et al., Mol. Cell 13 (1) :4-5 (2004) . Xia, L., et al., Cancer Res. 64 (1) :221-228 (2004) .