Dynamin Antibody

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002) . There are 3 known isofoms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin.

The antibody has been demonstrated to work in rat. It is also anticipated that the antibody will work with human, mouse, non-human primates, cow and chicken based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.

Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.

Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.