Ro52 Antibody

Product Specifications

NCBI Gene ID

6737

Swiss Prot

P19474

Accession Number

NP_003132.2

Host

Goat

Reactivity

Human

Immunogen

The immunogen for this antibody is: CPLNIGSQGSTDY

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Applications Notes

Peptide ELISA: Western Blot:Immunofluorescence: Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Additional validation:

Symbol

TRIM21

NCBI Official Name

Tripartite motif containing 21

NCBI Organism

Homo sapiens

Background Reference 01

Chan EK, Di Donato F, Hamel JC, Tseng CE, Buyon JP. 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart. J Exp Med. 1995 Oct 1;182 (4) :983-92.

Other Product Names

TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), tripartite motif protein TRIM21, tripartite motif protein 21