POLL Antibody

Product Specifications

NCBI Gene ID

27343

Swiss Prot

Q9UGP5

Accession Number

NP_037406.1

Host

Goat

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-LGLPYREPAERDW

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Positive Control

Cat. No. 1308 - Human Small Intestine Tissue Lysate

Concentration

500 ug/mL

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Applications Notes

Peptide ELISA: Western Blot:

Symbol

POLL

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

NCBI Official Name

Polymerase (DNA directed), lambda

NCBI Organism

Homo sapiens

Background Reference 01

Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem. 2002 Apr 12;277 (15) :13184-91.

Other Product Names

POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA polymerase lamda2, DNA-directed DNA polymerase lambda, OTTHUMP00000020323, pol beta2, pol lambda