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GAPDH Antibody

Product Specifications

NCBI Gene ID

2597

Swiss Prot

P04406

Accession Number

NP_002037.2, NP_001243728.1

Host

Goat

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-GVNHEKYDNSLK

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Positive Control

Cat. No. 1315 - Human Tonsil Tissue Lysate

Concentration

500 ug/mL

Homology

Expected Species Reactivity based on sequence homology: Dog

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1) . Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Applications Notes

Peptide ELISA: Western Blot:Immunohistochemistry:Immunofluorescence:

Symbol

GAPDH

NCBI Official Name

Glyceraldehyde-3-phosphate dehydrogenase

NCBI Organism

Homo sapiens

Background Reference 01

Ismail SA, Park HW. Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61 (Pt 11) :1508-13. Epub 2005 Oct 19

Other Product Names

GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

Tested Applications

ELISA, WB, IHC, IF

Protein ID

7669492

Physical Properties

Liquid

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