ChAT Antibody

Product Specifications

NCBI Gene ID

1103

Swiss Prot

P28329

Accession Number

NP_066264.3, NP_065574.3, NP_001136405.1

Host

Goat

Reactivity

Human

Immunogen

The immunogen for this antibody is: CKEKATRPSQGHQP

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Immunology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Positive Control

Cat. No. 1309 - Human Placenta Lysate

Concentration

500 ug/mL

Homology

Expected Species Reactivity based on sequence homology: Dog, Pig, Cow

Buffer

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and store at -20˚ C. Minimize freezing and thawing.

Specificity

This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1) . Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1) .

Applications Notes

Peptide ELISA: Western Blot:

Symbol

CHAT

NCBI Official Name

Choline O-acetyltransferase

NCBI Organism

Homo sapiens

Background Reference 01

Madziar B, Lopez-Coviella I, Zemelko V, Berse B. Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway. J Neurochem. 2005 Feb;92 (4) :767-79.