PRKAA2 Antibody

Product Specifications

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

NCBI Gene ID

5563

Swiss Prot

P54646

Accession Number

P54646

Host

Rabbit

Reactivity

Human

Immunogen

This PRKAA2 (Thr172) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human PRKAA2 (Thr172) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Neuroscience, Signal Transduction, Autophagy

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

62 kDa

Fragment

Rabbit Ig

Symbol

PRKAA2

NCBI Official Name

5'-AMP-activated protein kinase catalytic subunit alpha-2

NCBI Organism

Homo sapiens

Background Reference 01

Jablonski, K.A., et al. Diabetes 59 (10) :2672-2681 (2010)

Background Reference 02

Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

Background Reference 03

Jassim, G., et al. Pharmacopsychiatry (2010) In press :

Background Reference 04

Bungard, D., et al. Science 329 (5996) :1201-1205 (2010)

Other Product Names

5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2