GRP94 Antibody [SRPR90b]

Product Specifications

Background

Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

NCBI Gene ID

7184

Swiss Prot

P14625

Host

Rat

Reactivity

Human

Immunogen

Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Clonality

Monoclonal

Clone

SRPR90b

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2a, kappa

Applications Notes

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

HSP90B1

NCBI Official Name

Heat shock protein 90kDa beta (Grp94), member 1

NCBI Organism

Homo sapiens

Other Product Names

Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1