Cyclin D1 Antibody [CCND1/809]
Product Specifications
Background
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
NCBI Gene ID
595
Swiss Prot
P24385
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
Clonality
Monoclonal
Clone
CCND1/809
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Cell Cycle
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-460
Fragment
IgG2a, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
CCND1
NCBI Official Name
Cyclin D1
NCBI Organism
Homo sapiens
Other Product Names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
Tested Applications
Flow, IF, WB, IHC-P
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
