NP Antibody

Defects in NP are the cause of nucleoside phosphorylase deficiency (NP deficiency) . It leads to a severe T-cell immunodeficiency with neurologic disorder in children. The specific function of NP is not yet known.This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NP antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

NP antibody can be used for detection of NP by ELISA at 1:62500. NP antibody can be used for detection of NP by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.