TAF1A Antibody
Product Specifications
Background
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
NCBI Gene ID
9015
Swiss Prot
Q15573
Accession Number
NP_005672
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1A.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by Protein A/G affinity chromatography method.
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF1A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
53 kDa
Applications Notes
TAF1A antibody can be used for detection of TAF1A by ELISA at 1:15000. TAF1A antibody can be used for detection of TAF1A by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
TAF1A
NCBI Official Name
TATA box binding protein (TBP) -associated factor, RNA polymerase I, A, 48kDa
NCBI Organism
Homo sapiens
Background Reference 01
Di Pietro, C., et al., (2000) Cytogenet. Cell Genet. 89 (1-2) 133-136..
Other Product Names
TAF1A, SL1, RAFI48, TAFI48, MGC:17061
Tested Applications
ELISA, WB
Protein ID
5032143
Physical Properties
Liquid
Curated Selection
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