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TAF1A Antibody

Product Specifications

Background

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

NCBI Gene ID

9015

Swiss Prot

Q15573

Accession Number

NP_005672

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by Protein A/G affinity chromatography method.

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF1A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

53 kDa

Applications Notes

TAF1A antibody can be used for detection of TAF1A by ELISA at 1:15000. TAF1A antibody can be used for detection of TAF1A by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

TAF1A

NCBI Official Name

TATA box binding protein (TBP) -associated factor, RNA polymerase I, A, 48kDa

NCBI Organism

Homo sapiens

Background Reference 01

Di Pietro, C., et al., (2000) Cytogenet. Cell Genet. 89 (1-2) 133-136..

Other Product Names

TAF1A, SL1, RAFI48, TAFI48, MGC:17061

Tested Applications

ELISA, WB

Protein ID

5032143

Physical Properties

Liquid

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