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CHRNE Antibody

Product Specifications

Background

After binding acetylcholine, the ACHR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

NCBI Gene ID

1145

Swiss Prot

Q04844

Accession Number

NP_000071

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNE.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by Protein A/G affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHRNE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

55 kDa

Applications Notes

CHRNE antibody can be used for detection of CHRNE by ELISA at 1:375000. CHRNE antibody can be used for detection of CHRNE by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

CHRNE

NCBI Official Name

Cholinergic receptor, nicotinic, epsilon (muscle)

NCBI Organism

Homo sapiens

Background Reference 01

Beeson D.M.W, et al., (1993) Eur. J. Biochem. 215:229-238..

Other Product Names

CHRNE, ACHRE, CMS1D, CMS1E, CMS2A, FCCMS, SCCMS

Tested Applications

ELISA, WB

Protein ID

4557463

Physical Properties

Liquid

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