PCNA Antibody

PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PCNA antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

PCNA antibody can be used for detection of PCNA by ELISA at 1:62500. PCNA antibody can be used for detection of PCNA by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.