BCKDK Antibody
Product Specifications
Background
BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.
NCBI Gene ID
10295
Swiss Prot
O14874
Accession Number
NP_005872
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCKDK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store BCKDK antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
43 kDa
Applications Notes
BCKDK antibody can be used for detection of BCKDK by ELISA at 1:62500. BCKDK antibody can be used for detection of BCKDK by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
BCKDK
NCBI Official Name
Branched chain ketoacid dehydrogenase kinase
NCBI Organism
Homo sapiens
Background Reference 01
Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007) .
Other Product Names
BCKDK, BDK, BCKDKD
Tested Applications
ELISA, WB
Protein ID
171906589
Physical Properties
Liquid
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