PKLR Antibody
Product Specifications
Background
PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) .The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) . Alternatively spliced transcript variants encoding distinct isoforms have been described.
NCBI Gene ID
5313
Swiss Prot
P30613-2
Accession Number
NP_870986
Host
Rabbit
Reactivity
Human, Mouse, Rat, C. elegans, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PKLR.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PKLR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
58 kDa, 62 kDa
Applications Notes
PKLR antibody can be used for detection of PKLR by ELISA at 1:62500. PKLR antibody can be used for detection of PKLR by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PKLR
NCBI Official Name
Pyruvate kinase, liver and RBC
NCBI Organism
Homo sapiens
Background Reference 01
Pendergrass, D.C., (2006) IUBMB Life 58 (1), 31-38.
Other Product Names
PKLR, PK1, PKL, RPK, PKR, PKRL
Tested Applications
ELISA, WB, IHC
Protein ID
32967597
Physical Properties
Liquid
Curated Selection
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