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ILF3 Antibody

Product Specifications

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of ILF3.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

NCBI Gene ID

3609

Swiss Prot

Q12906-2

Accession Number

NP_004507

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ILF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

76 kDa, 95 kDa, 75 kDa

Applications Notes

ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be used for detection of ILF3 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

ILF3

NCBI Official Name

Interleukin enhancer binding factor 3, 90kDa

NCBI Organism

Homo sapiens

Background Reference 01

Nie, Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963.

Other Product Names

ILF3, CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, TCP110, MPHOSPH4, NF-AT-90

Tested Applications

ELISA, WB, IHC

Protein ID

24234753

Physical Properties

Liquid

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