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BRF1 Antibody

Product Specifications

Background

BRF1 is one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs.

NCBI Gene ID

72308

Swiss Prot

Q6PED6

Accession Number

AAH58112

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse BRF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store BRF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

22 kDa

Applications Notes

BRF1 antibody can be used for detection of BRF1 by ELISA at 1:62500. BRF1 antibody can be used for detection of BRF1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

BRF1

NCBI Official Name

BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)

NCBI Organism

Mus musculus

Background Reference 01

Strausberg, R.L., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903.

Other Product Names

BRF1, GTF3B, TAF3C, TAFIII90, TFIIIB90, mTFIIIB90, 2510002F24Rik

Tested Applications

ELISA, WB

Protein ID

34786067

Physical Properties

Liquid

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