PTRF Antibody

Product Specifications

Background

In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.

NCBI Gene ID

19285

Swiss Prot

O54724

Accession Number

NP_033012

Host

Rabbit

Reactivity

Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PTRF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

43 kDa

Applications Notes

PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.