AES Antibody

Product Specifications

Background

AES is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genesThe protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

166

Swiss Prot

Q08117

Accession Number

NP_001121

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AES.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AES antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

22 kDa, 29 kDa, 22 kDa

Applications Notes

AES antibody can be used for detection of AES by ELISA at 1:62500. AES antibody can be used for detection of AES by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.