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ILF2 Antibody

Product Specifications

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

NCBI Gene ID

3608

Swiss Prot

Q12905

Accession Number

NP_004506

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Signal Transduction, Immunology

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ILF2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

43 kDa

Applications Notes

ILF2 antibody can be used for detection of ILF2 by ELISA at 1:62500. ILF2 antibody can be used for detection of ILF2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

ILF2

NCBI Official Name

Interleukin enhancer binding factor 2

NCBI Organism

Homo sapiens

Background Reference 01

Warner C. Greene, et al., (2006) Proc Natl Acad Sci 103:15588.

Other Product Names

ILF2, NF45, PRO3063

Tested Applications

ELISA, WB, IHC

Protein ID

24234747

Physical Properties

Liquid

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