RNF2 Antibody

Product Specifications

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by RNF2 is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2) . Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

NCBI Gene ID

6045

Swiss Prot

Q99496

Accession Number

NP_009143

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1224 - Daudi Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RNF2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

38 kDa

Applications Notes

RNF2 antibody can be used for detection of RNF2 by ELISA at 1:312500. RNF2 antibody can be used for detection of RNF2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.