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PDLIM5 Antibody

Product Specifications

Background

PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

NCBI Gene ID

10611

Swiss Prot

Q96HC4

Accession Number

NP_001011513

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDLIM5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDLIM5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

54 kDa, 26 kDa, 24 kDa, 64 kDa

Applications Notes

PDLIM5 antibody can be used for detection of PDLIM5 by ELISA at 1:312500. PDLIM5 antibody can be used for detection of PDLIM5 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

PDLIM5

NCBI Official Name

PDZ and LIM domain 5

NCBI Organism

Homo sapiens

Background Reference 01

Lasorella, A., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (13), 4976-4981.

Other Product Names

PDLIM5, L9, ENH, LIM, ENH1

Tested Applications

ELISA, WB

Protein ID

374093201

Physical Properties

Liquid

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