PCAF Antibody

Product Specifications

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. PCAF associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

NCBI Gene ID

8850

Swiss Prot

Q92831

Accession Number

NP_003875

Host

Rabbit

Reactivity

Human, Mouse, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCAF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cell Cycle

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PCAF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

93 kDa

Applications Notes

PCAF antibody can be used for detection of PCAF by ELISA at 1:62500. PCAF antibody can be used for detection of PCAF by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.