EIF5 Antibody

Product Specifications

Background

EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .

NCBI Gene ID

1983

Swiss Prot

P55010

Accession Number

NP_892116

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

49 kDa

Applications Notes

EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.