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PIGO Antibody

Product Specifications

Background

PIGO is a protein that is involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. PIGO is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI.This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least two alternatively spliced transcripts encoding distinct isoforms have been found for this gene.

NCBI Gene ID

84720

Swiss Prot

B1AML3

Accession Number

NP_690577

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGO.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIGO antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

74 kDa

Applications Notes

PIGO antibody can be used for detection of PIGO by ELISA at 1:1562500. PIGO antibody can be used for detection of PIGO by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

PIGO

NCBI Official Name

Phosphatidylinositol glycan anchor biosynthesis, class O

NCBI Organism

Homo sapiens

Background Reference 01

Humphray, S.J., (2004) Nature 429 (6990), 369-374.

Other Product Names

PIGO, DKFZp434M222, FLJ00135, MGC20536, MGC3079, RP11-182N22.4, HPMRS2

Tested Applications

ELISA, WB

Protein ID

38045917

Physical Properties

Liquid

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