SF1 Antibody
Product Specifications
Background
SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. SF1 may act as transcription repressor.
NCBI Gene ID
7536
Swiss Prot
Q15637
Accession Number
NP_004621
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Tranfected 293T Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=25-438
Calculated Molecular Weight
68 kDa
Applications Notes
SF1 antibody can be used for detection of SF1 by ELISA at 1:312500. SF1 antibody can be used for detection of SF1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
SF1
NCBI Official Name
Splicing factor 1
NCBI Organism
Homo sapiens
Background Reference 01
Rino, J., (2008) Mol. Cell. Biol. 28 (9), 3045-3057.
Other Product Names
SF1, D11S636, ZFM1, ZNF162, BBP, MBBP, ZCCHC25
Tested Applications
ELISA, WB
Protein ID
42544130
Physical Properties
Liquid
Curated Selection
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