CLOCK Antibody

Product Specifications

Background

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.

NCBI Gene ID

9575

Swiss Prot

O15516

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 577-846 of human CLOCK (NP_004889.1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction, Transcription

Purification

Affinity purification

Positive Control

MCF7

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 70kDa

Fragment

IgG

Applications Notes

WB: 1:500 - 1:2000

Symbol

CLOCK

Positive Control 2

BT-474

Positive Control 3

NCI-H460

Positive Control 4

HepG2

Positive Control 5

BxPC-3

Positive Control 6

Mouse lung

NCBI Official Name

Clock circadian regulator

NCBI Organism

Homo sapiens

Other Product Names

CLOCK, clock homolog (mouse), KAT13D, bHLHe8, circadian locomoter output cycles kaput protein, circadian locomoter output cycles protein kaput, class E basic helix-loop-helix protein 8