EIF5B Antibody
Product Specifications
Background
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
NCBI Gene ID
9669
Swiss Prot
O60841
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Affinity purification
Positive Control
DU145
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 179kDa
Fragment
IgG
Applications Notes
WB: 1:500 - 1:2000
Symbol
EIF5B
Positive Control 2
HeLa
Positive Control 3
Jurkat
Positive Control 4
LO2
NCBI Official Name
Eukaryotic translation initiation factor 5B
NCBI Organism
Homo sapiens
Other Product Names
IF2, eukaryotic translation initiation factor 5B, eIF-5B, translation initiation factor IF-2, translation initiation factor IF2
Tested Applications
WB
Physical Properties
Liquid
Curated Selection
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