PCNA Antibody
Product Specifications
Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
NCBI Gene ID
5111
Swiss Prot
P12004
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Purification
Affinity purification
Positive Control
U-251MG
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 36kDa
Fragment
IgG
Applications Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:100
Symbol
PCNA
Positive Control 2
A-549
Positive Control 3
HL-60
Positive Control 4
HepG2
Positive Control 5
NIH/3T3
Positive Control 6
SW480
NCBI Official Name
Proliferating cell nuclear antigen
NCBI Organism
Homo sapiens
Other Product Names
PCNA, MGC8367
Tested Applications
WB, IHC
Physical Properties
Liquid
Curated Selection
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