KEAP1 Antibody

Product Specifications

Background

KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3) -dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) . KEAP1 also targets the down regulation of NF-κ B activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.

NCBI Gene ID

9817

Swiss Prot

Q14145

Accession Number

NP_987096

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

7045

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar KEAP1 expression profile in human, mouse (3T3/NIH) and rat (YB2/0) cell lines detected by two independent anti-KEAP1 antibodies that recognize different epitopes, 7045 against C-terminus domain and 22-002 against recombinant fusion protein of human KEAP1. KEAP1 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7045 KO Validation (Figure 3) : Anti-KEAP1 antibodies (7045) specificity was further verified by KEAP1 specific knockout. KEAP1 signal was not detected in KEAP1 knockout HeLa cells as compared to that in control wild type cells. KO Validation7045)

Field of Research

Signal Transduction

Purification

KEAP1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1302 - Human Lung Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%)

Buffer

KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

KEAP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of KEAP1 are known to exist.

Isoform

Human KEAP1 has 1 isoform, (624aa, 69.7kD) . Mouse KEAP1 has 1 isoform (624aa, 69.6kD) . Rat KEAP1 has only one isoform (624aa, 69.4 kD) . This antibody detects KEAP1 of human, mouse and rat.

Symbol

KEAP1

Positive Control 2

Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3

Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4

Cat. No. 1204– K562 Cell Lysate

NCBI Official Name

Kelch-like ECH-associated protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Zhang et al. Mol. Cell. Biol. 2004;24:10941-53.

Background Reference 02

Kobayashi et al. Mol. Cell. Biol. 2004; 24:7130-9.

Background Reference 03

Jiang et al. Int. J. Mol. Med. 2012; 29:946-56.

Background Reference 04

Devling et al. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A.

Other Product Names

KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2

Tested Applications

ELISA, WB, IF

Protein ID

45269145

Physical Properties

Liquid

Available Sizes

Curated Selection

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