PRC1 Antibody

Product Specifications

Background

PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs) . High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.

NCBI Gene ID

9055

Swiss Prot

O43663

Accession Number

NP_003972

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

PRC1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

Concentration

1 mg/mL

Buffer

PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PRC1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

Symbol

PRC1

NCBI Official Name

Protein regulator of cytokinesis 1

NCBI Organism

Homo sapiens

Background Reference 01

Jiang W, Jimenez G, Wells NJ, et al. PRC1: a human mitotic spindle-associated Cdk substrate protein required for cytokinesis. Mol. Cell 1998; 2:877-85.

Background Reference 02

Pellman D, Bagget M, Tu H, et al. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces Cerevisiae. J. Cell Biol. 1995; 130:1373-85.

Background Reference 03

Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004; 23:3237-48.

Background Reference 04

Mollinari C, Kleman JP, Jiang W, et al. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 2002; 157:1175-86.