DDA Antibody

Product Specifications

Background

DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.

NCBI Gene ID

79016

Swiss Prot

Q9BW61

Accession Number

NP_003253

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

DDA Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1401 - Mouse Heart Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)

Buffer

DDA Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DDA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

DDA1

NCBI Official Name

DET1 and DDB1 associated 1

NCBI Organism

Homo sapiens

Background Reference 01

Pick E, Lau O, Tsuge T, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol. Cell. Biol. 2007; 27:4708-19.

Background Reference 02

Olma MH, Roy M, Le Bihan T, et al. An interaction network of the mammalian COP9 signalsome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J. Cell Sci. 2009; 122:1035-44