GEMC1 Antibody

Product Specifications

Background

GEMC1 Antibody: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.

NCBI Gene ID

647309

Swiss Prot

A6NCL1

Accession Number

NP_001140158

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

GEMC1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1401 - Mouse Heart Tissue Lysate

Concentration

1 mg/mL

Buffer

GEMC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GEMC1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

GEMC1

NCBI Official Name

Geminin coiled-coil domain-containing protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Piergiovanni G, Costanzo V. GEMC1 is a novel TopBP1-interacting protein involved in chromosomal DNA replication. Cell Cycle 2010; 9:3662-6.

Background Reference 02

McGarry TJ and Kirschner MW. Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 1998; 93:1043-53.

Background Reference 03

Rowles A and Blow JJ. Chromatin proteins involved in the initiation of DNA replication. Curr. Opin. Genet. Dev. 1997; 7:152-7.

Background Reference 04

Balestrini A, Cosentino C, Errico A, et al. GEMC1 is a TopBP1 interacting protein required for chromosomal DNA replication. Nat. Cell Biol. 2010; 12:484-91.