KLOTHO Antibody

KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

Independent Antibody Validation in Cell lines (Figure 1) shows similar KLOTHO expression profile in human and mouse cell lines detected by two independent anti-KLOTHO antibodies that recognize different epitopes, 6107 against central domain and competitor antibody against human recombinant protein. KLOTHO proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6107 competitor antibody Recombinant Protein Test (Figure 2) : Anti-KLOTHO antibodies (6107) detected human KLOTHO recombinant protein at different concentrations. Recombinant Protein Test6107

KLOTHO antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human KLOTHO has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD) . Mouse KLOTHO has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD) . Rat KLOTHO has one isoform (1014aa, 117kD) . 6107 can detect human, mouse and rat.