SCF Antibody

SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Recombinant Protein Test (Figure 2) : Anti-SCF antibodies (5165) detected human SCF recombinant protein at different concentrations. Recombinant Protein Test (5165) Regulated expression validation (Figure 7 & 8) : SCF expression detected by anti-SCF antibodies (5165) was up-regulated by angiotensin II (Ang II) treatment and was down-regulated by two inhibitors (Figure 7) or CNP treatment (Figure 8) . Regulated expression validation (5165)

SCF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD) . Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD) . Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD) . 5165 can detect human and possibly detect mouse and rat.