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Alpha-tubulin Antibody

Product Specifications

Background

Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.

NCBI Gene ID

7278

Swiss Prot

Q13748

Accession Number

NP_005992

Host

Chicken

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Alpha-tubulin Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1463 - Rat Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%)

Buffer

Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Alpha-tubulin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=5103

Fragment

IgY

Symbol

TUBA3C

NCBI Official Name

Tubulin, alpha 3c

NCBI Organism

Homo sapiens

Background Reference 01

McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci.2001; 114:2723-33.

Background Reference 02

Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol.1988; 2:133-53.

Background Reference 03

Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem.2009; 109:683-693.

Other Product Names

Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2

Tested Applications

ELISA, WB

Protein ID

17921993

Physical Properties

Liquid

Available Sizes

Curated Selection

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