TEM1 Antibody

Product Specifications

Background

TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.

NCBI Gene ID

57124

Swiss Prot

Q9HCU0

Accession Number

NP_065137

Host

Rabbit

Reactivity

Human

Immunogen

4357

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain. TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4357 4359

Field of Research

Homeostasis, Cancer

Purification

TEM1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1320 - Human Colon Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse (86%) ; Rat: (86%)

Buffer

TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TEM1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.

Isoform

Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD) . Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD) . 4357 can detect all isoforms of human, mouse and rat.

Symbol

CD248

Positive Control 2

Cat. No. 1223 - Caco-2 Cell Lysate

Positive Control 3

Cat. No. 1219 – MCF-7 Cell Lysate

Positive Control 4

Cat. No. 1220 – SK-N-SH Cell Lysate

NCBI Official Name

CD248 molecule, endosialin

NCBI Organism

Homo sapiens

Background Reference 01

Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.

Background Reference 02

Christian et al. J. Biol. Chem. 2001; 276:7408-14.

Background Reference 03

Nanda et al. Curr. Opin. Oncol. 2004; 16:44-9.

Background Reference 04

Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.

Other Product Names

TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

9966885

Physical Properties

Liquid

Available Sizes

Curated Selection

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