MANF Antibody

Product Specifications

Background

MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

NCBI Gene ID

7873

Swiss Prot

P55145

Accession Number

P55145

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

4349

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Recombinant Protein Test (Figure 2) : Anti-MANF antibodies (4349) detected human MANF recombinant protein at different concentrations. Recombinant Protein Test4349 Induced Expression Validation (Figure 6) : MANF expression detected by anit-MANF antibodies was up-regulated by VPA treatment. Induced Expression Validation

Field of Research

Neuroscience

Purification

MANF Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1463 - Rat Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (92%)

Buffer

MANF Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

MANF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This antibody does not cross-react with CDNF.

Isoform

Human MANF has one isoform (182aa, 21kD) . Mouse MANF has one isoform (179aa, 20kD) and Rat MANF also has one isoform (179aa, 20kD) . 4349 can detect human, mouse and rat.

Symbol

MANF

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Mesencephalic astrocyte derived neurotrophic factor

NCBI Organism

Homo sapiens

Background Reference 01

Shridhar et al. Oncogene 1996; 12:1931-9.

Background Reference 02

Shridhar et al. Cancer Res. 1996; 56:5576-8.

Background Reference 03

Petrova et al. J. Mol. Neurosci. 2003; 20:173-88.

Background Reference 04

Lindholm et al. Nature2007; 448:73-7.

Citation 01

24460582

Citation 02

22243807

Other Product Names

MANF Antibody: ARP, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

23503040

Physical Properties

Liquid

Available Sizes

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