MAPKAP1 Antibody

Product Specifications

Background

MAPKAP1 Antibody: MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.

NCBI Gene ID

79109

Swiss Prot

Q9BPZ7

Accession Number

NP_001006618

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

MAPKAP1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (100%)

Buffer

MAPKAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

MAPKAP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

MAPKAP1

NCBI Official Name

Mitogen-activated protein kinase associated protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Schroder W, Cloonan N, Bushell G, et al. Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene. Gene2004; 339:17-23.

Background Reference 02

Cheng J, Zhang D, Kim K, et al. Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation. Mol. Cell. Biol.2005; 25:5955-64.

Background Reference 03

Jacinto E, Facchinetti V, Liu D, et al. SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell2006; 127:125-37.

Background Reference 04

Wullschleger S, Loewith R and Hall MN. TOR signaling in growth and metabolism. Cell2006; 124:471-84.

Other Product Names

MAPKAP1 Antibody: MIP1, SIN1, JC310, SIN1b, SIN1g, MIP1, Target of rapamycin complex 2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, TORC2 subunit MAPKAP1