DRAM Antibody

DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Independent Antibody Validation in Cell lines (Figure 2) shows similar DRAM expression profile in human, mouse and rat cell lines detected by two independent anti-DRAM antibodies that recognize different epitopes, 4033 against C-terminus domain and 4035 against N-terminus domain. DRAM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4033 4035 Induced Expression Validation (Figure 5) : DRAM expression detected by anit-DRAM antibodies (4033) was up-regulated by CQ treatment in WT neural precursor cells. Induced Expression Validation4033 Regulated expression validation (Figure 6) : DRAM expression detected by anit-DRAM antibodies (4033) was down-regulated by in WT neural precursor cells. Regulated expression validation4033

DRAM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human DRAM has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (128aa, 14kD) . Mouse DRAM also has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (132aa, 15kD) . Rat DRAM has only one isoform (238aa, 26kD) . 4033 can detect human, mouse and rat isoforms.