PDCD4 Antibody

Product Specifications

Background

PDCD4 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

NCBI Gene ID

27250

Swiss Prot

Q53EL6

Accession Number

NP_663314

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

PDCD4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (100%)

Buffer

PDCD4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PDCD4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

PDCD4

Positive Control 2

Cat. No. 1403 - Mouse Brain Tissue Lysate

NCBI Official Name

Programmed cell death 4 (neoplastic transformation inhibitor)

NCBI Organism

Homo sapiens

Background Reference 01

Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.

Background Reference 02

Shibihara K, Asano M, Ishida Y, et al. Isolation of a novel mouse gene MA-3 that is induced upon programmed cell death. Gene 1995; 166:297-301.

Background Reference 03

Goke R, Barth P, Schmidt A, et al. Programmed cell death 4 suppresses CDK1/cdc2 via induction of p21 (Waf1/Cip1) . Am. J. Physiol. Cell Physiol. 2004; 287:C1541-6.

Background Reference 04

Yang HS, Jansen AP, Nair R, et al. The transformation suppressor Pdcd4 is a novel translation initiation factor 4A binding protein that inhibits translation. Mol. Cell Biol. 2003; 23:26-37.