PIKE Antibody

Product Specifications

Background

PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

NCBI Gene ID

116986

Swiss Prot

Q99490

Accession Number

AAM97540

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

PIKE Antibody is Ion exchange chromatography purified.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (86%)

Buffer

PIKE Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PIKE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.

Symbol

AGAP2

NCBI Official Name

ArfGAP with GTPase domain, ankyrin repeat and PH domain 2

NCBI Organism

Homo sapiens

Background Reference 01

Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30.

Background Reference 02

Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95.

Background Reference 03

Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61.

Background Reference 04

Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer) -A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51.