BACE Antibody

BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) . BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Independent Antibody Validation in Cell lines (Figure 2) shows similar BACE expression profile in human cell lines detected by two independent anti-BACE antibodies that recognize different epitopes, 2253 against C-terminus domain and 32-238 against recombinant fragment protein. BACE proteins are detected in all the tested cell lines except K562 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2253 KO validation (Figure 6,9) : Anti-BACE antibody (2253) specificity was further verified by BACE KO mice (figure6) and KO cell line (figure9) . BACE signal was not detected in BACE KO mice and KO cell line. KO validation2253 KD validation (Figure 7,8,10) : Anti-BACE antibody (2253) specificity was verified by BACE specific siRNA knockdown. BACE signal in mouse brain injected with BACE siRNAs and DRG transfected with BACE siRNAs was disrupted in comparison with control. KD validation2253 Overexpression validation (Figure 6) : Anti-BACE antibody (2253) detected high expression levels of BACE in 293 cells transfected with hBACE or mBACE (figure6) as compared to eGFP transfected cells. Overexpression validation2253

BACE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human BACE has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD) . This antibody detects all human isoforms. Mouse BACE has only 1 isoform (501aa, 55.7kD) . Rat BACE has only one isoform identified so far (501aa, 55.8kD) .